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HHHHHHconjugated to KLH.
Our Abpromise guarantee covers the use of ab9108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21483675|
|ICC/IF||1/100 - 1/500.|
|ChIP||Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.|
|Flow Cyt||Use at an assay dependent concentration.
(see Abreview published on 22nd January, 2007).
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
This image is courtesy of an anonymous Abreview
ab9108 staining CACNB3 with His tag expressed in HEK293 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% goat serum, 0.1% TX100 and PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/750).
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