PE Anti-6X His tag®抗体[AD1.1.10] (ab72467)
Key features and details
- PE Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
PE Anti-6X His tag®抗体[AD1.1.10]
参阅全部 6X His tag® 一抗 -
描述
PE小鼠单克隆抗体[AD1.1.10] to 6X His tag® -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide HHHHHH tag - tagged
-
常规说明
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 1.64% Sodium phosphate, 1.71% Sucrose, 0.1% BSA, 0.87% Sodium chloride -
Concentration information loading...
-
纯度
Size exclusion -
纯化说明
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
克隆
单克隆 -
克隆编号
AD1.1.10 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72467于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | (2) |
1/50 - 1/100.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
Flow Cyt
1/50 - 1/100. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
-
BALB-3T3 cells, which express moderate amounts of EGFR, were incubated with a His6-tagged anti-EGFR fibronectin domain. The labeled cells were washed and incubated with 50 microliters of 1:15 diluted PE-conjugated anti-His6 antibody. Cells were washed and analyzed by flow cytometry. A control without fibronectin domain was also analyzed. Image: Dashed black line is control without fibronectin. Solid blue line is sample with His6-tagged anti-EGFR fibronectin domain.
数据表及文件
-
SDS download
-
Datasheet download
文献 (13)
ab72467 被引用在 13 文献中.
- Yanakieva D et al. Beyond bispecificity: Controlled Fab arm exchange for the generation of antibodies with multiple specificities. MAbs 14:2018960 (2022). PubMed: 35014603
- Burke MJ et al. A bovine antibody possessing an ultralong complementarity-determining region CDRH3 targets a highly conserved epitope in sarbecovirus spike proteins. J Biol Chem 298:102624 (2022). PubMed: 36272646
- Herrera NG et al. Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis. ACS Omega 6:85-102 (2021). PubMed: 33458462
- Kalimuddin S et al. Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset. Med (N Y) 2:682-688.e4 (2021). PubMed: 33851143
- Herrera NG et al. Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis. bioRxiv N/A:N/A (2020). PubMed: 32587972