Anti-6X His tag®抗体[AD1.1.10] (DyLight® 650) (ab117504)

概述

  • 产品名称Anti-6X His tag®抗体[AD1.1.10] (DyLight® 650)
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[AD1.1.10] to 6X His tag® (DyLight® 650)
  • 偶联物DyLight® 650. Ex: 654nm, Em: 673nm
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 免疫原

    Synthetic peptide corresonding to 6X His tag®.

  • 常规说明Stable for 1yr.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号AD1.1.10
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504)参考文献

ab117504 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells transfected with His-tagged actin)
Permeabilization Yes - 0.1% Triton-X100 in PBS
Specification HeLa cells transfected with His-tagged actin
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
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提交于 Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"