Anti-6X His tag®抗体[AD1.1.10] (ab81663)

概述

  • 产品名称
    Anti-6X His tag®抗体[AD1.1.10]
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[AD1.1.10] to 6X His tag®
  • 特异性
    This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. It reacts against all His tagged proteins so far tested.
  • 经测试应用
    适用于: WB, IP, ELISAmore details
  • 免疫原

    PAX6 transcription factor linked to histidine tag.

  • 常规说明
    HIS-TAG® is a trademark of EMD Biosciences, Inc.


    The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. In Western blotting of bacterial extracts clone ADI.I.10 has been shown not to cross react with any endogenous products, although some cross reactivity may be seen with extracts of insect or mammalian cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. In Western blotting of bacterial extracts clone ADI.I.10 has been shown not to cross react with any endogenous products, although some cross reactivity may be seen with extracts of insect or mammalian cells.
  • 克隆
    单克隆
  • 克隆编号
    AD1.1.10
  • 骨髓瘤
    NS1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

文献

This product has been referenced in:
  • Kaspar J  et al. A unique open reading frame within the comX gene of Streptococcus mutans regulates genetic competence and oxidative stress tolerance. Mol Microbiol 96:463-82 (2015). Read more (PubMed: 25620525) »

See 1 Publication for this product

客户评价及客户问答

I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

Important aspects for his tag recognition are, the localisation of the tag (N- o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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