概述

  • 产品名称Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗
  • 描述
    山羊多克隆抗体to 6X His tag®
  • 特异性This antibody was verified by ELISA against peptide conjugated to BSA(HHHHHH/BSA).
  • 经测试应用适用于: ELISA, ICC/IF, ICC, WBmore details
  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.

  • 常规说明HIS-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 22247289
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody 图像

  • ab9136, staining 6X His tag® in VMP001-conjugated nanoparticles by Immunocytochemistry/ Immunofluorescence. An AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.

Anti-6X His tag® antibody (ab9136)参考文献

This product has been referenced in:
  • Fischer F  et al. Identification of potential mitochondrial CLPXP protease interactors and substrates suggests its central role in energy metabolism. Sci Rep 5:18375 (2015). WB . Read more (PubMed: 26679294) »
  • Moon JJ  et al. Enhancing humoral responses to a malaria antigen with nanoparticle vaccines that expand Tfh cells and promote germinal center induction. Proc Natl Acad Sci U S A 109:1080-5 (2012). ICC/IF . Read more (PubMed: 22247289) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver)
Permeabilization No
Specification Liver
Blocking step BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 26 2016

Here is the answer to your question: I'm afraid the antibody has not been tested for its ability to recognize internal epitopes, sorry about the inconvenience.

Thank you for your enquiry. To our knowledge, these antibodies have yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you get...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"