Anti-6X His tag®抗体(ab9107)

概述

  • 产品名称Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗
  • 描述
    鸡多克隆抗体to 6X His tag®
  • 特异性Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
  • 经测试应用适用于: WB, ELISAmore details
  • 免疫原

    Synthetic peptide:

    HHHHHH

    conjugated to KLH.

  • 常规说明HIS-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 纯度IgY fraction
  • 纯化说明Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP.
    The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding.
  • 靶标

    • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
    • 别名
      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    Anti-6X His tag® antibody (ab9107)参考文献

    ab9107 has not yet been referenced specifically in any publications.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"