Anti-6X His tag®抗体(ab125262)

概述

  • 产品名称Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗
  • 描述
    兔多克隆抗体to 6X His tag®
  • 特异性This antibody recognizes His tagged fusion proteins. It is a tag antibody.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 免疫原

    A synthetic peptide of HHHHHH conjugated to a carrier protein.

  • 阳性对照
    • His tagged fused proteins.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody 图像

  • All lanes : Anti-6X His tag® antibody (ab125262) at 0.5 µg/ml

    Lane 1 : His tagged recombinant protein (N terminal tag)
    Lane 2 : His tagged recombinant protein (N terminal tag)
    Lane 3 : His tagged recombinant protein (C terminal tag)

Anti-6X His tag® antibody (ab125262)参考文献

ab125262 has not yet been referenced specifically in any publications.

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