The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml.
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
6 His epitope tag antibody
Hexa His tag antibody
HHHHHH epitope tag antibody
HHHHHH tag antibody
His tag antibody
Polyhistidine Tag antibody
Anti-6X His tag® antibody 图像
Western blot - Anti-6X His tag® antibody (ab125262)
All lanes : Anti-6X His tag® antibody (ab125262) at 0.5 µg/ml
Lane 1 : His tagged recombinant protein (N terminal tag) Lane 2 : His tagged recombinant protein (N terminal tag) Lane 3 : His tagged recombinant protein (C terminal tag)
Anti-6X His tag® antibody (ab125262)参考文献
has not yet been referenced specifically in any publications.