Anti-6X His tag®抗体[4D11] (ab5000)


  • 产品名称Anti-6X His tag®抗体[4D11]
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[4D11] to 6X His tag®
  • 特异性This antibody recognises all proteins which contain either an N or C-terminal HHHHHHGS epitope tag. It has been shown by a reviewer to recognise HHHHHH alone.
  • 经测试应用适用于: Dot blot, ELISA, WB, IP, ICC/IF, Other, Flow Cytmore details
  • 免疫原

    Recombinant malaria protein TRAP fused at the amino-terminal end with six His tag. The coding sequence of TRAP 1.0 was amplified from P. falciparum genomic DNA (ITO4 isolate) in standard Polymerase Chain Reaction PCR (Saiki et al. 1988) using the primer combination trap 1 5'-AGTTGGATCCAGAGATGTGCAAAACA ATATAGTGG-3' and trap 2 5'-GCGAGTAAAGCTAAGCTTCCAGCTATTCCACCTGC-3', the amplified DNA sequence was cloned in the vector pDS56/RBSII,6xHis (Hochuli et al. 1988).

  • 表位This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).
  • 阳性对照
  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.


    Alternative versions available:

    Anti-6X His tag® antibody (HRP) [4D11] (ab202004)


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号4D11
  • 骨髓瘤NS1
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab5000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
AP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/1000.
IP Use at an assay dependent concentration. See Abreview.
ICC/IF Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [4D11] 图像

  • All lanes : Anti-6X His tag® antibody [4D11] (ab5000) at 1/2000 dilution

    Lane 1 : Lysates prepared from HEK-293 cells
    Lane 2 : Lysates prepared from HEK-293 cells transfected with Beta3-His (CACNB3)

    HRP-conjugated goat anit-mouse IgG polyclonal at 1/5000 dilution

    This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

    See Abreview

  • ab5000 Immunoprecipitate in human HEK293 whole cell lysate. 25µg of cell lysate incubated with primary antibody (undiluted) and matrix (Protein G) for 16 hours at 4°C. For western blotting an undiluted HRP-Goat anti-rabbit IgG polyclonal was used. Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab

    Lane 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)

    Lane 2. IP with anti His Ab

    Lane 3. Non bound fraction

    See Abreview

  • Anti-6X His tag® antibody [4D11] (ab5000) at 1/1000 dilution + 6X His Tagged Protein at 1 µg

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds
  • ab5000 staining Human HEK 293 cells transfected with CACNB3-His by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody (1/250 in 1% goat serum, 0.1% TX100, 1X PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody (1/500) was used as the secondary.  DAPI was used for nuclei staining (blue).

    See Abreview

Anti-6X His tag® antibody [4D11] (ab5000)参考文献

This product has been referenced in:
  • Zhang J  et al. DAO1 catalyzes temporal and tissue-specific oxidative inactivation of auxin in Arabidopsis thaliana. Proc Natl Acad Sci U S A 113:11010-5 (2016). WB . Read more (PubMed: 27651492) »
  • Turpin J  et al. Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses. J Virol 89:931-51 (2015). WB, ICC/IF . Read more (PubMed: 25355890) »

See all 12 Publications for this product

Product Wall

The main difference between ab18184 and ab5000 is the immunogen used to produce the antibodies. ab18184 was produced against a 6X His synthetic peptide, while ab5000 was raised against a recombinant protein fused to a 6X His tag. Thus, ab18184 was orig...

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Thank you for contacting us.

I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

Important aspects for his tag recognition ...

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Thank you for contacting us. The laboraotry sent the response below. Please let me know if you have any further questions.

I really wouldn't expect the antibody would cross-react with glycine, particularly as there is only a single glycine in ...

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Thank you for contacting us.

Please check the catalogue number ab5000;


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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab18184

To check the status of the order please contact our Customer Se...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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Thank you for contacting us and your interest in our products.

The antibodies you are interested in are provided following different purification steps. The ab84041 is provides as concentrated culture supernatant, ab5000 is provides as IgG fr...

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Thank you for your reply.

As requested, a free of charge replacementwith ab18184 has been added to your next order, number *******.

I am sorry to hear the suggestions made have not improved the results on this occasion.
Unfortunately, the only lot of ab5000 we have in stock is the same as the one your customer received. I would be pleased to arrange a replacement with an a...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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