Anti-6X His tag®抗体[3H2201] (Biotin) (ab15145)

概述

  • 产品名称Anti-6X His tag®抗体[3H2201] (Biotin)
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[3H2201] to 6X His tag® (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    In Western Blot of bacterial extracts, the antibody has been shown not to crossreact with any endogenous products. Some Reactivity may be seen with extracts of insect or mammalian cells.
  • 免疫原

    His-His-His-His-His-His conjugated to KLH.

  • 常规说明HIS-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号3H2201
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000.
ELISA 1/500 - 1/1000.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [3H2201] (Biotin) (ab15145)参考文献

ab15145 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply message. I am sorry to confirm that the biotin binding of ab15145 has not been quantified, but it is typically in the range of 4-6 biotin molecules / antibody. The biotin is linked through lysine residues on the antibody s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"