Anti-6X His tag®抗体[3H2201] (ab15149)

概述

  • 产品名称Anti-6X His tag®抗体[3H2201]
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[3H2201] to 6X His tag®
  • 经测试应用适用于: ICC/IF, IHC-Fr, IP, WBmore details
  • 种属反应性
    It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to react with any endogenous products, although some Reactivity may be seen with extracts of insect or mammalian cells.
  • 免疫原

    PAX6 transcription factor linked to histidine tag.

  • 常规说明HIS-TAG® is a trademark of EMD Biosciences, Inc.


    ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Tissue cuture supernatant purified by Protein G affinity chromatography.
  • Primary antibody说明ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.
  • 克隆单克隆
  • 克隆编号3H2201
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/100.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [3H2201] (ab15149)参考文献

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

Important aspects for his tag recognition ...

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Thank you for contacting us and your interest in our products.

The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other qu...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. Even though ab15149 has not been tested in ELISA, since the previous lot worked okay we will offer a replace...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. Please can you clarify how you have been applying this antibody and the...

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Thank you for your enquiry. Since you're looking for a 6X His tag antibody that works in both Western blotting and IHC, I would recommend ab15149. This antibody has been tested for application in Western blotting and Immunohistochemistry (Frozen sectio...

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Thank you for your enquiry and your interest in our products. This antibody recognises proteins containing the motif HHHHHH (5x His) and it is therefore of value in detecting proteins containing histidine tags (either at the N- or C-terminal). The a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"