Anti-6X His tag®抗体[33D10.D2] (Biotin) (ab106261)

概述

  • 产品名称
    Anti-6X His tag®抗体[33D10.D2] (Biotin)
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[33D10.D2] to 6X His tag® (Biotin)
  • 偶联物
    Biotin
  • 特异性
    ab106261 detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino or carboxy-termini of targeted proteins in transfected or transformed cells.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 免疫原

    6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

  • 常规说明

    10-20 BAC molecules per Mouse Monoclonal Antibody

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    33D10.D2
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
ELISA 1/200000.

靶标

  • 相关性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

实验方案

Anti-6X His tag® antibody [33D10.D2] (Biotin) (ab106261)参考文献

ab106261 has not yet been referenced specifically in any publications.

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