Anti-6X His tag®抗体(ab14923)
Key features and details
- Rabbit polyclonal to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-6X His tag®抗体
参阅全部 6X His tag® 一抗 -
描述
兔多克隆抗体to 6X His tag® -
宿主
Rabbit -
特异性
ab14923 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus in transfected or transformed cells.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
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阳性对照
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein, ab2431.
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常规说明
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14923于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 0.5 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 µg/ml. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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Anti-6X His tag® antibody (ab14923) at 0.6 µg/ml +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab14923 被引用在 15 文献中.
- Wan J et al. MYB70 modulates seed germination and root system development in Arabidopsis. iScience 24:103228 (2021). PubMed: 34746697
- Zhang X et al. SIZ1-Mediated SUMO Modification of SEUSS Regulates Photomorphogenesis in Arabidopsis. Plant Commun 1:100080 (2020). PubMed: 33367258
- Yang L et al. PIF1 and RVE1 form a transcriptional feedback loop to control light-mediated seed germination in Arabidopsis. J Integr Plant Biol 62:1372-1384 (2020). PubMed: 32277734
- Yang L et al. Interplay between REVEILLE1 and RGA-LIKE2 regulates seed dormancy and germination in Arabidopsis. New Phytol 225:1593-1605 (2020). PubMed: 31580487
- Salari S et al. Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice. Parasit Vectors 13:415 (2020). PubMed: 32787908