Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell)
Loading amount
20 µg
Specification
neuronal cell
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 0.5% skim milk
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Additional data
Abcam response
This review notes the primary antibody was used 'undiluted'. This is unlikely however further user information is unfortunately unavailable.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 09 2010