Application
IHC - Wholemount
Sample
Xenopus tropicalis Embryo (whole embryo)
Specification
whole embryo
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: PBS+ 0.1% Triton
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anri-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
The antibody did not work in Xenopus tropicalis.
Abcam response
Thank you for submitting your feedback on this untested application. While the antibody should work for any species that has a 6X His tag, we have not tested for IHC Wholemount. Whole mount tissue or mbryos is much thicker than a standard tissue section and may need additional time for blocking and incubation. We recommend a primary antibody incubation of 48 hours for the primary antibody for optimal staining. Please let us know if you have any furhter questions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 04 2014