Anti-68kDa Neurofilament抗体- Neuronal Marker (ab9035)
- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
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产品名称Anti-68kDa Neurofilament抗体- Neuronal Marker
参阅全部 68kDa Neurofilament 一抗 -
描述兔多克隆抗体to 68kDa Neurofilament - Neuronal Marker
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宿主Rabbit
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特异性Specifically recognizes the light neurofilament subunit (~68 kDa).
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经测试应用适用于: WB, IHC-FoFr, IHC-P, IHC-Fr, ICC/IFmore details
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种属反应性与反应: Rat, Pig
预测可用于: Bird, Mammal
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免疫原
Pig intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and this cytoskeletal material was dissolved in 6M urea. NF-L was purified by ion exchange chromatography, followed by preparative gel electrophoresis.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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纯度Whole antiserum
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab9035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IHC-FoFr | ||
| IHC-P | ||
| IHC-Fr | ||
| ICC/IF |
应用说明
ICC/IF: 1/500.
IHC-F: 1/1000.
IHC-P: 1/1000.
IHC-Fr: 1/1000.
WB: 1/5000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-F: 1/1000.
IHC-P: 1/1000.
IHC-Fr: 1/1000.
WB: 1/5000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
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疾病相关Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
序列相似性Belongs to the intermediate filament family.
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结构域The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
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翻译后修饰O-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 100521224 Pig
- Entrez Gene: 83613 Rat
- SwissProt: P02547 Pig
- SwissProt: P19527 Rat
- Unigene: 18568 Rat
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别名
- 68 kDa neurofilament protein antibody
- 68kDa neurofilament protein antibody
- CMT1F antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament antibody - Neuronal Marker (ab9035)ab9035 staining anti 68kDa Neurofilament in mixed neuron/glia cultures from newborn rat brain by ICC/IF (Immunocytochemistry/immunofluorescence). Samples were incubated with primary antibody (1/500) (red) and co-stained with ab4573 for anti Peripherin (green).
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Western blot - Anti-68kDa Neurofilament antibody - Neuronal Marker (ab9035)Anti-68kDa Neurofilament antibody - Neuronal Marker (ab9035) at 1/20000 dilution + whole rat cerebellum homogenate
A prominent band at ~68kDa corresponds to rodent NF-L. Human, cow and bovine NF-L run a little slower, at about 70kDa. -
Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament antibody - Neuronal Marker (ab9035)This image is courtesy of an Abreview submitted by Dr Luis Craveiroab9035 at a 1/300 dilution staining rat hippocampal organotypic slice cultures by ICC/IF. The primary antibody was incubated with the cells for 120 hours (this time allows for the antibody to penetrate a layer of glial cells that grows while the slice is in culture). Bound antibody is detected using a Cy3 conjugated Indocarbocyanine.
实验方案
文献
This product has been referenced in:
- Yadav P et al. Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling. Acta Neuropathol 132:93-110 (2016). WB ; Mouse . Read more (PubMed: 27021905) »
- Clarke WT et al. Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons. J Cell Sci : (2010). WB ; Mouse . Read more (PubMed: 20587592) »
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