Anti-68kDa Neurofilament抗体[DA2] (ab7255)

概述

  • 产品名称Anti-68kDa Neurofilament抗体[DA2]
    参阅全部 68kDa Neurofilament 一抗
  • 描述
    小鼠单克隆抗体[DA2] to 68kDa Neurofilament
  • 特异性Specifically recognizes the light neurofilament subunit or NF-L (~68 kDa).
  • 经测试应用适用于: Flow Cyt, ICC/IF, WB, IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Bird, all Mammals
  • 免疫原

    A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immungen followed by immunofluorescence microscopy.

  • 表位The epitope for this antibody is not in the N-terminus; however exactly where it is located is not known. It is thought to be somewhere within the central alpha helical "rod" region of the molecule.

性能

应用

Our Abpromise guarantee covers the use of ab7255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.
ICC/IF 1/100 - 1/500.
WB 1/5000 - 1/10000.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. (works on mildly formalin fixed sections)
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • 疾病相关Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • 序列相似性Belongs to the intermediate filament family.
  • 结构域The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • 翻译后修饰O-glycosylated.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 68 kDa neurofilament protein antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all

Anti-68kDa Neurofilament antibody [DA2] 图像

  • Lane 1 : Coomassie Blue stain
    Lane 2 : Heavy neurofilament antibody
    Lane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)
    Lane 4 : Anti-68kDa Neurofilament antibody [DA2] (ab7255)
    Lane 5 : alpha Internexin antibody
    Lane 6 : GFAP antibody

    Lane 1 : Cytoskeletal homogenates of rat spinal cord
    Lane 2 : Cytoskeletal homogenates of rat spinal cord
    Lane 3 : Cytoskeletal homogenates of rat spinal cord
    Lane 4 : Cytoskeletal homogenates of rat spinal cord
    Lane 5 : Cytoskeletal homogenates of rat spinal cord
    Lane 6 : Cytoskeletal homogenates of rat spinal cord
  • IHC image of ab7255 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7255, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing SH-SY5Y cells stained with ab7255 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7255, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-68kDa Neurofilament antibody [DA2] (ab7255)参考文献

This product has been referenced in:
  • Rupprecht A  et al. Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. PLoS One 9:e88474 (2014). WB ; Mouse . Read more (PubMed: 24523901) »
  • Roher AE  et al. Subjects harboring presenilin familial Alzheimer's disease mutations exhibit diverse white matter biochemistry alterations. Am J Neurodegener Dis 2:187-207 (2013). Read more (PubMed: 24093083) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. The membrane used was 0.45micron nitrocellulose, blocked in 5% non fat milk in TBS, antibody incubations in TBS +0.1% tween 20, ab7255 conc was equivalent of 1:500 from our standard tissue culture supernatant prep and i...

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Dear Sir/Madam: Our anti-NF-L monoclonal antibody (catalog# NB 300-132) isunpurified mouse ascites (50 ul). We suugest a working dilution of 1:1,000 for ECL. Best Regards, Kate Lynott

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"