Anti-68kDa Neurofilament抗体(ab24520)

概述

  • 产品名称
    Anti-68kDa Neurofilament抗体
    参阅全部 68kDa Neurofilament 一抗
  • 描述
    鸡多克隆抗体to 68kDa Neurofilament
  • 宿主
    Chicken
  • 经测试应用
    适用于: IHC, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cat, Human
  • 免疫原

    Tissue/ cell preparation (Cow): bovine intermediate filaments prepared from spinal cords.

  • 阳性对照
    • ICC/IF: SKNSH cells. WB: Rat brain and spinal cord lysates; mouse brain and spinal cord lysates; cow spinal cord lysate. IHC: Rat cerebellum tissue.
  • 常规说明


    To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. To ensure greater specificity for NFL, animals were boosted with recombinant mouse NFL purified from bacteria. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.

性能

应用

Our Abpromise guarantee covers the use of ab24520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC 1/2000.
ICC/IF 1/5000.
WB 1/10000. Predicted molecular weight: 63 kDa.

Using chemiluminescence, 1/10000 or lower.

靶标

  • 功能
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • 疾病相关
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 结构域
    The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • 翻译后修饰
    O-glycosylated.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 68 kDa neurofilament protein antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all

图片

  • ICC/IF image of ab24520 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24520, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-68kDa Neurofilament antibody (ab24520) at 1/20000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat spinal cord lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse spinal cord lysate
    Lane 5 : Cow spinal cord lysate

    Predicted band size: 63 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed (transcardial perfusion) of free floating section of rat cerecellum tissue stained for 68kDa Neurofilament (green) using ab24520 at 1/200 dilution. FOX3/NeuN is stained with an anti-FOX3/NeuN antibody (red).

文献

ab24520 has not yet been referenced specifically in any publications.

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