Anti-68kDa Neurofilament抗体(ab113854)

概述

  • 产品名称
    Anti-68kDa Neurofilament抗体
    参阅全部 68kDa Neurofilament 一抗
  • 描述
    兔多克隆抗体to 68kDa Neurofilament
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Horse, Chicken, Cow, Dog, Pig, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human 68kDa Neurofilament.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Rat Cerebellum; Human Spinal Cord; Mouse Spinal Cord.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
  • 疾病相关
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
    Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 结构域
    The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
  • 翻译后修饰
    O-glycosylated.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 68 kDa neurofilament protein antibody
    • 68kDa neurofilament protein antibody
    • CMT1F antibody
    • CMT2E antibody
    • FLJ53642 antibody
    • Light molecular weight neurofilament protein antibody
    • NEFL antibody
    • Neurofilament light antibody
    • Neurofilament light polypeptide 68kDa antibody
    • Neurofilament light polypeptide antibody
    • Neurofilament protein, light chain antibody
    • Neurofilament subunit NF L antibody
    • Neurofilament triplet L protein antibody
    • NF-L antibody
    • NF68 antibody
    • NFL antibody
    • NFL_HUMAN antibody
    see all

Anti-68kDa Neurofilament antibody 图像

  • All lanes : Anti-68kDa Neurofilament antibody (ab113854) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Cerebellum Rat Tissue Lysate
    Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

实验方案

Anti-68kDa Neurofilament antibody (ab113854)参考文献

ab113854 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample
Chicken Tissue sections (spinal cord)
Specification
spinal cord
Permeabilization
Yes - PBS + 0.1% Tween 20
Fixative
Paraformaldehyde
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提交于 Feb 24 2014

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