Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
25 µg
Specification
Mouse brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk tbst
Dilution
1/10000
Secondary antibody
Dilution
1/100000
Name
Non-Abcam antibody was used: Sigma Anti chicken IgY
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Ecl plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 68 kDa
Positive control
This sample
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 24 2012