Anti-67kDa Laminin Receptor抗体(ab137388)
Key features and details
- Rabbit polyclonal to 67kDa Laminin Receptor
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-67kDa Laminin Receptor抗体
参阅全部 67kDa Laminin Receptor 一抗 -
描述
兔多克隆抗体to 67kDa Laminin Receptor -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig, Monkey -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-294 of Human 67kDa Laminin Receptor (Uniprot ID: P08865).
-
阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells; Human CA922 xenograft tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137388于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/1000.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
|
说明 |
---|
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
靶标
-
功能
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. -
序列相似性
Belongs to the ribosomal protein S2P family. -
翻译后修饰
Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions. -
细胞定位
Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 281898 Cow
- Entrez Gene: 3921 Human
- Entrez Gene: 100505031 Mouse
- Entrez Gene: 16785 Mouse
- Entrez Gene: 641351 Pig
- Entrez Gene: 29236 Rat
- Omim: 150370 Human
- SwissProt: P26452 Cow
see all -
别名
- 34/67 kDa laminin receptor antibody
- 37 kDa laminin receptor precursor antibody
- 37/67 kDa laminin receptor antibody
see all
图片
-
Anti-67kDa Laminin Receptor antibody (ab137388) at 1/1000 dilution + HepG2 whole cell lysate
at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Anti-67kDa Laminin Receptor antibody (ab137388) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labelling 67kDa Laminin Receptor with ab137388 at 1/200 dilution. Lower image is merged with DNA probe.
-
Immunohistochemical analysis of paraffin-embedded Human CA922 xenograft tissue, labelling 67kDa Laminin Receptor with ab137388 at 1/500 dilution.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab137388 被引用在 5 文献中.
- Della Via FI et al. (-)-Epigallocatechin-3-gallate induces apoptosis and differentiation in leukaemia by targeting reactive oxygen species and PIN1. Sci Rep 11:9103 (2021). PubMed: 33907248
- Jiang X et al. Control of ribosomal protein synthesis by the Microprocessor complex. Sci Signal 14:N/A (2021). PubMed: 33622983
- Garita-Hernandez M et al. AAV-Mediated Gene Delivery to 3D Retinal Organoids Derived from Human Induced Pluripotent Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32028585
- Ashok A et al. Prion protein modulates endothelial to mesenchyme-like transition in trabecular meshwork cells: Implications for primary open angle glaucoma. Sci Rep 9:13090 (2019). PubMed: 31511544
- Khajuria RK et al. Ribosome Levels Selectively Regulate Translation and Lineage Commitment in Human Hematopoiesis. Cell 173:90-103.e19 (2018). PubMed: 29551269