6-Phosphofructokinase Activity Assay试剂盒(Colorimetric) (ab155898)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 1 mU/well
概述
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产品名称
6-Phosphofructokinase Activity Assay试剂盒(Colorimetric) -
检测方法
Colorimetric -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Enzyme activity -
灵敏度
< 1 mU/well -
范围
2 nmol/well - 10 nmol/well -
检测时间
1h 30m -
种属反应性
与反应: Mammals, Other species -
产品概述
6-Phosphofructokinase Activity Assay kit ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.
In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
PFK activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K776 Phosphofructokinase (PFK) Activity Colorimetric Assay Kit. K776-100 is the same size as the 100 test size of ab155898.
Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).
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平台
Microplate reader
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer VII 1 x 27ml ATP III 1 vial CK Enzyme Mix 1 vial Developer Solution III 1 vial NADH Standard I 1 vial PFK Positive Control 1 vial PFK Substrate 1 vial -
研究领域
图片
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab155898 被引用在 22 文献中.
- Khurana P et al. Maternal Undernutrition Induces Cell Signalling and Metabolic Dysfunction in Undifferentiated Mouse Embryonic Stem Cells. Stem Cell Rev Rep 19:767-783 (2023). PubMed: 36517693
- Sahasakul Y et al. Partial Substitution of Glucose with Xylitol Prolongs Survival and Suppresses Cell Proliferation and Glycolysis of Mice Bearing Orthotopic Xenograft of Oral Cancer. Nutrients 14:N/A (2022). PubMed: 35631164
- Yoo HJ et al. MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation. Cell Rep 41:111598 (2022). PubMed: 36351405
- Haythorne E et al. Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells. Nat Commun 13:6754 (2022). PubMed: 36376280
- Koh JH et al. Enhancement of anaerobic glycolysis - a role of PGC-1a4 in resistance exercise. Nat Commun 13:2324 (2022). PubMed: 35484130