概述

  • 产品名称Anti-5T4抗体
    参阅全部 5T4 一抗
  • 描述
    小鼠单克隆抗体to 5T4
  • 经测试应用适用于: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 219-329 of Human 5T4, with a 26 KDa tag (NP_006661).

  • 阳性对照
    • HeLa and PC12 cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab88091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 24736661ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 24736661

靶标

  • 组织特异性Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
  • 序列相似性Contains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 形式5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60 (Ward et al., 2006).
  • 别名
    • 5T4 antibody
    • 5T4 oncofetal antigen antibody
    • 5T4 oncofetal trophoblast glycoprotein antibody
    • 5T4 oncotrophoblast glycoprotein antibody
    • 5T4AG antibody
    • AW495680 antibody
    • M6P1 antibody
    • TPBG antibody
    • TPBG_HUMAN antibody
    • Trophoblast glycoprotein antibody
    see all

Anti-5T4 antibody 图像

  • Anti-5T4 antibody (ab88091) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Anti-5T4 antibody (ab88091) at 5 µg/ml + PC12 cell lysate at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Detection limit for ab88091 is approximately 1ng/ml as a capture antibody.

Anti-5T4 antibody (ab88091)参考文献

This product has been referenced in:
  • Patterson KG  et al. Control of established colon cancer xenografts using a novel humanized single chain antibody-streptococcal superantigen fusion protein targeting the 5T4 oncofetal antigen. PLoS One 9:e95200 (2014). IF, Flow Cyt ; Human . Read more (PubMed: 24736661) »

See 1 Publication for this product

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