Anti-5HT7 Receptor抗体(ab61562)

概述

  • 产品名称Anti-5HT7 Receptor抗体
    参阅全部 5HT7 Receptor 一抗
  • 描述
    兔多克隆抗体to 5HT7 Receptor
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Marmoset (common), Bat
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to 19 amino acids from the 3rd cytoplasmic domain of Human 5HT7 Receptor.

  • 阳性对照
    • Human brain cortex tissue.

性能

应用

Our Abpromise guarantee covers the use of ab61562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/200.

靶标

  • 功能This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
  • 组织特异性Isoform A is the predominant isoform in spleen, caudate and hippocampus. Isoform B is expressed at lower levels. Isoform D is a minor isoform in term of expression.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 HT 7 antibody
    • 5 HT X antibody
    • 5 hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase coupled) antibody
    • 5 hydroxytryptamine (serotonin) receptor 7 antibody
    • 5 hydroxytryptamine receptor 7 antibody
    • 5-HT-7 antibody
    • 5-HT-X antibody
    • 5-HT7 antibody
    • 5-hydroxytryptamine receptor 7 antibody
    • 5HT7 antibody
    • 5HT7R_HUMAN antibody
    • htr7 antibody
    • Serotonin 5 HT 7 receptor antibody
    • Serotonin receptor 7 antibody
    see all

Anti-5HT7 Receptor antibody 图像

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue, staining 5HT7 Receptor with ab61562 at 10 µg/ml.
  • ICC/IF image of ab61562 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61562, 1:200) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-5HT7 Receptor antibody (ab61562)参考文献

ab61562 has not yet been referenced specifically in any publications.

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