概述

  • 产品名称Anti-5HT6 Receptor抗体
    参阅全部 5HT6 Receptor 一抗
  • 描述
    兔多克隆抗体to 5HT6 Receptor
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse 5HT6 Receptor.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse dorsal root ganglion; Mouse brain P0; Mouse brain P7. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Brain.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. It has a high affinity for tricyclic psychotropic drugs.
  • 组织特异性Expressed in several human brain regions, most prominently in the caudate nucleus.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 HT 6 antibody
    • 5 HT6 antibody
    • 5 HT6R antibody
    • 5 hydroxytryptamine (serotonin) receptor 6 G protein coupled antibody
    • 5 hydroxytryptamine 6 receptor antibody
    • 5 hydroxytryptamine receptor 6 antibody
    • 5-HT-6 antibody
    • 5-HT6 antibody
    • 5-hydroxytryptamine receptor 6 antibody
    • 5HT 6 antibody
    • 5HT6R_HUMAN antibody
    • HTR 6 antibody
    • Htr6 antibody
    • Serotonin receptor 6 antibody
    see all

Anti-5HT6 Receptor antibody 图像

  • IHC image of 5HT6 Receptor staining in Mouse Brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103016, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-5HT6 Receptor antibody (ab103016) at 1 µg/ml

    Lane 1 : Dorsal Root Ganglia (Mouse) Tissue Lysate
    Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 3 : P7 Mouse Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    5HT6 Receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

Anti-5HT6 Receptor antibody (ab103016)参考文献

This product has been referenced in:
  • Jacobshagen M  et al. The serotonin 6 receptor controls neuronal migration during corticogenesis via a ligand-independent Cdk5-dependent mechanism. Development 141:3370-7 (2014). WB, IHC (PFA fixed) ; Mouse . Read more (PubMed: 25078650) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest in ab103016.

To our knowledge, ab103016 has not been tested in ICC/IF. We do not have any small aliquots for testing. Therefore, I can offer a discount off a future purchase if you buy ab103016 now, test...

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Merci pour votre réponse.
J'ai comme convenu mis en place l'envoi d'une unité d'anti-5HT6 ab101912. Le numéro de commande de remplacement gratuit de ce produit : *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'e...

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Merci de nous avoir contactés et désolé pour le délai de notre réponse.
J'ai contacté mes collègues du laboratoire, ils n'ont malheureusement pas testé cet anticorps avec un contrôle négatif. Les tests de caractérisation montrent une bande à ˜55 kD...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"