概述

  • 产品名称Anti-5HT3B receptor抗体
    参阅全部 5HT3B receptor 一抗
  • 描述
    兔多克隆抗体to 5HT3B receptor
  • 特异性Specific to 5HT3B - does not recognise 5HT3A receptor This antibody recognizes the intracellular region of the 5HT3B subunit.
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 320-338 of Human 5HT3B receptor (large intracellular loop region)

性能

应用

Our Abpromise guarantee covers the use of ab39629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500.
IP Use at an assay dependent concentration.

靶标

  • 功能This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • 组织特异性Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta.
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily.
  • 翻译后修饰N-glycosylation required for membrane localization.
  • 细胞定位Cell membrane. Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 HT3 B antibody
    • 5 HT3B antibody
    • 5 HTR3B antibody
    • 5 hydroxytryptamine 3 receptor B subunit antibody
    • 5 hydroxytryptamine receptor 3B antibody
    • 5 hydroxytryptamine serotonin receptor 3B antibody
    • 5-HT3-B antibody
    • 5-HT3B antibody
    • 5-hydroxytryptamine receptor 3B antibody
    • 5HT3B_HUMAN antibody
    • 5HTR3B antibody
    • Htr3b antibody
    • Serotonin gated ion channel subunit antibody
    • Serotonin receptor 3B antibody
    see all

Anti-5HT3B receptor antibody 图像

  • All lanes : Anti-5HT3B receptor antibody (ab39629) at 1/500 dilution

    Lane 1 : Lysate from HEK 293 cells
    Lane 2 : Lysate from HEK 293 cells transfected with 5HT3A receptor
    Lane 3 : Lysate from HEK 293 cells transfected with 5HT3A receptor and 5HT3B receptor
    Lane 4 : Human hippocampus lysate


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • HEK 293 cells transfected with 5HT3B receptor were stained with ab39629 diluted 1/500 and anti-rabbit secondary antibody conjugated to Alexa Fluor 488 diluted 1/1000(green). Nuclei are labelled with Hoescht 33258 (blue).
  • Immunohistochemistry of human hippocampus stained with ab39269 diluted 1/500. Immunoreactivity with 5HT3B receptor can be seen in (i)CA1 field, (ii)CA2, (iii)CA3 and (iv)CA4/dentate gyrus.

Anti-5HT3B receptor antibody (ab39629)参考文献

This product has been referenced in:

See 1 Publication for this product

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Thank you very much for your interest in ab39629.
To our knowledge, ab39629 has not been tested in flow cytometry. Therefore, I can offer a discount off a future purchase if you buy ab39629 now, test it in flow cytometry and submit feedback to us ...

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Thank you for contacting us. We would normally recommend using a rabbit IgG and use the same donkey anti-rabbit FITC secondary in order to detect any interaction. Your Rabbit IgG Isotype control-FITC should be applicable but may cause some differenc...

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