Anti-5HT2C Receptor抗体(ab32887)

概述

  • 产品名称Anti-5HT2C Receptor抗体
    参阅全部 5HT2C Receptor 一抗
  • 描述
    山羊多克隆抗体to 5HT2C Receptor
  • 特异性Ab32887 recognises 5HT2C Receptor.
  • 经测试应用适用于: WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-QVENLELPVN

    , corresponding to amino acids 437-446 of Human 5HT2C Receptor.

  • 阳性对照
    • Lysates of EBV immortalised Lymphoblastoids, T cell, MOLT4 and monocytic cell line U937.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).Can be blocked with 5HT2C Receptor peptide (ab168550).
ELISA 1/64000.
IHC-Fr Use at an assay dependent concentration. Abreview 30686 suggests that this antibody is not suitable for this application

靶标

  • 功能This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 结构域The PDZ domain-binding motif is involved in the interaction with MPDZ.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 Hydroxytryptamine 2C receptor antibody
    • 5-HT-1C antibody
    • 5-ht-1c receptor antibody
    • 5-HT-2C antibody
    • 5-HT1C antibody
    • 5-HT2C antibody
    • 5-HTR2C antibody
    • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
    • 5-hydroxytryptamine receptor 1C antibody
    • 5-hydroxytryptamine receptor 2C antibody
    • 5HT1C antibody
    • 5HT2C antibody
    • 5HT2C_HUMAN antibody
    • 5HTR2C antibody
    • 5Hydroxytryptamine 2C receptor antibody
    • Htr1c antibody
    • HTR2C antibody
    • serotonin 1c receptor antibody
    • serotonin 2c receptor antibody
    • Serotonin 5-HT-2C receptor antibody
    • Serotonin receptor 2C antibody
    see all

Anti-5HT2C Receptor antibody 图像

  • Anti-5HT2C Receptor antibody (ab32887) at 1 µg/ml + EBV immortalised Lymphoblastoid lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

Anti-5HT2C Receptor antibody (ab32887)参考文献

ab32887 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

Great news! The blocking peptide has been added as 5HT2C Receptor peptide ab168550.

http://www.abcam.com/5ht2c-receptor-peptide-ab168550.html

I hope this information helps. Please contact us wit...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative formalin
Antigen retrieval step Enzymatic
Permeabilization Yes - 1xPBT
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C
Username

Abcam user community

Verified customer

提交于 Sep 04 2012


I am very pleased to hear you would like to accept our offer and test ab32887 and ab44640 in IHC-FoFr and WB, respectively. These codes will give you: 1 free primary antibody each before the expiration date. To redeem this offer, please submit an ...

Read More
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (hypothalamus PVN sections)
Specification hypothalamus PVN sections
Fixative ethanol 70%
Permeabilization Yes - triton-x
Blocking step 2% BSA 2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Ms. Rebecca Ring

Verified customer

提交于 Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"