概述

  • 产品名称
    Anti-5HT1B Receptor抗体
    参阅全部 5HT1B Receptor 一抗
  • 描述
    兔多克隆抗体to 5HT1B Receptor
  • 特异性
    This antibody has been sucessfully used on rat tissue in IHC. Since the immunogen only shares 84% homology, customers may get variable results. Please see the abreview for more details.
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human, Common marmoset
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human 5HT1B Receptor.

    (Peptide available as ab94829.)

  • 阳性对照
    • This antibody gave a positive signal in human brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab85937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰
    Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.
    Palmitoylated.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 HT1DB antibody
    • 5 hydroxytryptamine receptor 1B antibody
    • 5-HT-1B antibody
    • 5-HT-1D-beta antibody
    • 5-HT1B antibody
    • 5-hydroxytryptamine receptor 1B antibody
    • 5HT1B_HUMAN antibody
    • Htr1b antibody
    • HTR1D2 antibody
    • HTR1DB antibody
    • S12 antibody
    • Serotonin 1D beta receptor antibody
    • Serotonin receptor 1B antibody
    see all

图片

  • 5HT1B Receptor antibody at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 20 minutes
  • ab85937 staining 5HT1B Receptor on Marmoset Hippocampus (upper) and Cortex (lower). The tissue were fixed in formaldehyde and subjeted to heated mediated antigen retrieval uisng citric acid (pH 6). the tissue was blocked by incubating in 1% BSA for 10 mins at 21°C. The antibody was diluted 1/500 using TBS/BSA/Azide. The antibody was incubated for 2 hours at 21°C.

    See Abreview

文献

This product has been referenced in:
  • Kratz A  et al. Digital expression profiling of the compartmentalized translatome of Purkinje neurons. Genome Res 24:1396-410 (2014). Read more (PubMed: 24904046) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Hippocampus)
Specification
Hippocampus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 06 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Marmoset brain: Cortex)
Specification
Marmoset brain: Cortex
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Aug 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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