Anti-5HT3A receptor/5ht3抗体(ab51950)
Key features and details
- Goat polyclonal to 5HT3A receptor/5ht3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-5HT3A receptor/5ht3抗体
参阅全部 5HT3A receptor/5ht3 一抗 -
描述
山羊多克隆抗体to 5HT3A receptor/5ht3 -
宿主
Goat -
特异性
ab51950 is expected to recognise both reported isoforms (NP_998786.1 and NP_000860.1). -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human 5HT3A receptor/5ht3 aa 390-401 (internal sequence).
Sequence:CGPQDFEKSPRDR
Database link: P46098 -
阳性对照
- Flow Cyt (Intra): HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.05% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab51950 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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说明 |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
靶标
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功能
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. -
组织特异性
Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. -
细胞定位
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3359 Human
- Omim: 182139 Human
- SwissProt: P46098 Human
- Unigene: 413899 Human
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别名
- 5 HT 3 antibody
- 5 HT3 receptor antibody
- 5 HT3A antibody
see all
图片
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Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) labelling 5HT3A receptor with ab51950. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
数据表及文件
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Datasheet download
文献 (2)
ab51950 被引用在 2 文献中.
- Yamada J et al. PSA-NCAM Colocalized with Cholecystokinin-Expressing Cells in the Hippocampus Is Involved in Mediating Antidepressant Efficacy. J Neurosci 40:825-842 (2020). PubMed: 31801810
- Li F et al. Shuyu Capsules Relieve Premenstrual Syndrome Depression by Reducing 5-HT3AR and 5-HT3BR Expression in the Rat Brain. Neural Plast 2016:7950781 (2016). PubMed: 27725889