Anti-5HT3A receptor/5ht3抗体(ab13897)
Key features and details
- Rabbit polyclonal to 5HT3A receptor/5ht3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-5HT3A receptor/5ht3抗体
参阅全部 5HT3A receptor/5ht3 一抗 -
描述
兔多克隆抗体to 5HT3A receptor/5ht3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat 5HT3A receptor/5ht3. The immunogen is a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE).
Database link: P35563 -
阳性对照
- human brain lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13897于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa. |
靶标
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功能
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. -
组织特异性
Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. -
细胞定位
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3359 Human
- Entrez Gene: 15561 Mouse
- Entrez Gene: 79246 Rat
- Omim: 182139 Human
- SwissProt: P46098 Human
- SwissProt: P23979 Mouse
- SwissProt: P35563 Rat
- Unigene: 413899 Human
see all -
别名
- 5 HT 3 antibody
- 5 HT3 receptor antibody
- 5 HT3A antibody
see all
图片
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All lanes : Anti-5HT3A receptor/5ht3 antibody (ab13897) at 3 µg/ml
Lane 1 : Human Brain tissue lysate
Lane 2 : Mouse Brain tissue lysate
Lane 3 : Rat Brain tissue lysate
Predicted band size: 55 kDa -
IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western Blot of human brain lysate staining 5HT3A receptor with ab13897
数据表及文件
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Datasheet download
文献 (9)
ab13897 被引用在 9 文献中.
- Chang WY et al. 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome. Lab Invest 102:1023-1037 (2022). PubMed: 35585132
- Kameneva P et al. Serotonin limits generation of chromaffin cells during adrenal organ development. Nat Commun 13:2901 (2022). PubMed: 35614045
- Correia AS et al. Serotonin Type 3 Receptor Is Potentially Involved in Cellular Stress Induced by Hydrogen Peroxide. Life (Basel) 12:N/A (2022). PubMed: 36295079
- Zhu Y et al. Effects of sacral nerve electrical stimulation on 5-HT and 5-HT3AR/5-HT4R levels in the colon and sacral cord of acute spinal cord injury rat models. Mol Med Rep 22:763-773 (2020). PubMed: 32626986
- Cheng Q et al. [6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT3 Receptor System in Rats. Drug Des Devel Ther 14:4085-4099 (2020). PubMed: 33061309
- Fu YL et al. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochem Biophys Res Commun 511:356-362 (2019). PubMed: 30791981
- Li B et al. Deciphering the multicomponent synergy mechanisms of SiNiSan prescription on irritable bowel syndrome using a bioinformatics/network topology based strategy. Phytomedicine 63:152982 (2019). PubMed: 31299593
- Zhao L et al. Prolongation of bronchopulmonary C-fiber-mediated apnea by prenatal nicotinic exposure in rat pups: role of 5-HT3 receptors. FASEB J 33:10731-10741 (2019). PubMed: 31251077
- Nguyen H et al. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterol Motil 20:228-35 (2008). WB ; Rat . PubMed: 17919312