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Our Abpromise guarantee covers the use of ab19072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.03 - 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).|
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 18315599|
ab19072 at 3.8µg/ml staining 58K Golgi protein in human liver tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded tissue sections). Steamed antigen retrieval in citrate buffer pH 6 was performed. AP staining procedure was used for detection.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"