Anti-58K Golgi protein抗体(ab19072)

概述

  • 产品名称Anti-58K Golgi protein抗体
    参阅全部 58K Golgi protein 一抗
  • 描述
    山羊多克隆抗体to 58K Golgi protein
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide:

    SQLVECVPNFSEGKNQ

    , corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein.

  • 阳性对照
    • Human liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab19072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.03 - 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 18315599

靶标

  • 功能Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
    Binds and promotes bundling of vimentin filaments originating from the Golgi.
  • 通路Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
    One-carbon metabolism; tetrahydrofolate interconversion.
  • 疾病相关Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
  • 序列相似性In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
    In the N-terminal section; belongs to the formiminotransferase family.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Formimidoyltetrahydrofolate cyclodeaminase antibody
    • Formimidoyltransferase cyclodeaminase antibody
    • Formiminotetrahydrofolate cyclodeaminase antibody
    • Formiminotransferase cyclodeaminase antibody
    • Formiminotransferase-cyclodeaminase antibody
    • FTCD antibody
    • FTCD_HUMAN antibody
    • Glutamate formiminotransferase antibody
    • Glutamate formyltransferase antibody
    • LCHC 1 antibody
    • LCHC1 antibody
    see all

Anti-58K Golgi protein antibody 图像

  • Anti-58K Golgi protein antibody (ab19072) at 0.03 µg/ml + tissue lysates prepared from human liver

    Secondary
    HRP-conjugated anti goat IgG

    Predicted band size : 59 kDa


    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab19072 at 3.8µg/ml staining 58K Golgi protein in human liver tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded tissue sections). Steamed antigen retrieval in citrate buffer pH 6 was performed. AP staining procedure was used for detection.

Anti-58K Golgi protein antibody (ab19072)参考文献

This product has been referenced in:
  • Schrage YM  et al. Aberrant heparan sulfate proteoglycan localization, despite normal exostosin, in central chondrosarcoma. Am J Pathol 174:979-88 (2009). IHC-P ; Human . Read more (PubMed: 19179614) »
  • Willems SM  et al. Myxoid tumours of soft tissue: the so-called myxoid extracellular matrix is heterogeneous in composition. Histopathology 52:465-74 (2008). IHC-P ; Human . Read more (PubMed: 18315599) »

See all 3 Publications for this product

Product Wall

Thank you for send the question again. You can use the antibodies with the secondary, however be advised that we have only tested tissue sections and not cell cultures, though I would expect it is likely to work for cells as well. Please let me know if...

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Unfortunately none of our Golgi markers have been tested against protists. Sorry I couldn't help you more,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"