Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SK-N-SH)
Specification
SK-N-SH
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Other product details
Concentration
5 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a strong golgi-associated staining pattern in interphase cells. MeOH fixed samples were also evaluated and also produced a similar staining pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Feb 02 2012