概述

  • 产品名称Anti-53BP2/ASPP2抗体
    参阅全部 53BP2/ASPP2 一抗
  • 描述
    兔多克隆抗体to 53BP2/ASPP2
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide mapping to a region between residue 425 and 475 of human 53BP2/ASPP2 using the numbering given in entry NP_001026855.1

  • 阳性对照
    • Whole cell lysate from HeLa or 293T cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab70548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 125 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
  • 组织特异性Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
  • 序列相似性Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • 结构域The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
  • 细胞定位Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 53BP2 antibody
    • Apoptosis stimulating of p53 protein 2 antibody
    • Apoptosis stimulating protein of p53 2 antibody
    • Apoptosis-stimulating of p53 protein 2 antibody
    • ASPP2 antibody
    • ASPP2_HUMAN antibody
    • BBP antibody
    • Bcl2 binding protein antibody
    • Bcl2-binding protein antibody
    • NY REN 51 antigen antibody
    • p53 binding protein 2 antibody
    • p53-binding protein 2 antibody
    • p53BP2 antibody
    • PPP1R13A antibody
    • Renal carcinoma antigen NY-REN-51 antibody
    • Tp53bp2 antibody
    • Tumor protein p53 binding protein 2 antibody
    • Tumor suppressor p53 binding protein 2 antibody
    • Tumor suppressor p53-binding protein 2 antibody
    see all

Anti-53BP2/ASPP2 antibody 图像

  • All lanes : Anti-53BP2/ASPP2 antibody (ab70548) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 125 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70548 at 3ug/mg of lysate(lane 1) or a control IgG (lane 2). Fr the subsequent blot, 20% of immnunoprecipitate was loaded per lane, and probed with ab70548 at 1ug/ml. Detection: chemiluminescence with exposure time of 30 seconds
  • IHC image of ab70548 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70548, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-53BP2/ASPP2 antibody (ab70548)参考文献

ab70548 has not yet been referenced specifically in any publications.

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