概述

  • 产品名称Anti-53BP1 (phospho S25)抗体
    参阅全部 53BP1 一抗
  • 描述
    兔多克隆抗体to 53BP1 (phospho S25)
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Phosphorylated synthetic peptide corresponding to a portion of 53BP1 around serine 25 (NP_005648.1)

  • 阳性对照
    • Human colon adenocarcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab82550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • 疾病相关Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • 序列相似性Contains 2 BRCT domains.
  • 翻译后修饰Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 (phospho S25) antibody 图像

  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (mock phosphatase treated section), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (lambda and CIP phosphatase treated), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue,

Anti-53BP1 (phospho S25) antibody (ab82550)参考文献

This product has been referenced in:
  • Roossink F  et al. The role of ATM and 53BP1 as predictive markers in cervical cancer. Int J Cancer 131:2056-66 (2012). IHC-P ; Human . Read more (PubMed: 22323184) »

See 1 Publication for this product

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