Anti-53BP1 (phospho S25)抗体(ab70323)

概述

  • 产品名称
    Anti-53BP1 (phospho S25)抗体
    参阅全部 53BP1 一抗
  • 描述
    兔多克隆抗体to 53BP1 (phospho S25)
  • 经测试应用
    适用于: WB, IP, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A phosphorylated synthetic peptide representing a portion of human 53BP1 around serine 25 (NP_005648.1.)

  • 阳性对照
    • Whole cell lysate from 293T cells treated with NCS (200 ng/ml for 1h).

性能

应用

Our Abpromise guarantee covers the use of ab70323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately >220 kDa (predicted molecular weight: 214 kDa).
IP Use at 1-4 µg/mg of lysate.
Flow Cyt Use 0.25µg for 106 cells.

0.25 μg per 1 million cells in a 150 mcl reaction.





ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • 疾病相关
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • 序列相似性
    Contains 2 BRCT domains.
  • 翻译后修饰
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • 细胞定位
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse teratoma (right) tissues labelling 53BP1 (phospho S24) with ab70323 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-53BP1 (phospho S25) antibody (ab70323) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells, untreated
    Lane 2 : Whole cell lysate from 293T cells treated with NCS (200 ng/ml for 1h)

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 214 kDa
    Observed band size : >220 kDa (why is the actual band size different from the predicted?)

文献

ab70323 has not yet been referenced specifically in any publications.

客户评价及客户问答

These are very similar antibodies.

The ab82550 Anti-53BP1 (phospho S25) antibody is reformulated to specifically cater to needs expressed by IHC specialists to have antibodies that can be used between a 1:100 and 1:500 dilution. Both are teste...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 1mM EDTA, 0.05% Tween-20
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 03 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Aug 20 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole embryos (~10 embryos per lane))
Loading amount
200000 cells
Specification
whole embryos (~10 embryos per lane)
Treatment
X-rays (0, 25 and 50 Gy)
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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