概述

  • 产品名称Anti-53BP1抗体
    参阅全部 53BP1 一抗
  • 描述
    兔多克隆抗体to 53BP1
  • 特异性This antibody is specific for 53BP1 protein.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide representing a portion of human 53BP1 encoded exon 19 (LocusLink ID 7158).

  • 阳性对照
    • U205, 293T and MO59K cell lysates (human). MEF lysates (mouse).

性能

应用

Our Abpromise guarantee covers the use of ab36828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
ICC/IF 1/200 - 1/1000.

靶标

  • 功能May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • 疾病相关Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • 序列相似性Contains 2 BRCT domains.
  • 翻译后修饰Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 antibody 图像

  • Anti-53BP1 antibody (ab36828) at 1/2000 dilution + Whole cell lysate from human U205 cells at 20 µg
    Developed using the ECL technique

    Predicted band size : 214 kDa
    Observed band size : 220 kDa (why is the actual band size different from the predicted?)

Anti-53BP1 antibody (ab36828)参考文献

This product has been referenced in:
  • Han W  et al. Nitric oxide mediated DNA double strand breaks induced in proliferating bystander cells after alpha-particle irradiation. Mutat Res 684:81-9 (2010). ICC/IF ; Chinese Hamster . Read more (PubMed: 20026341) »

See 1 Publication for this product

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