Anti-53BP1抗体(ab36823)
Key features and details
- Rabbit polyclonal to 53BP1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-53BP1抗体
参阅全部 53BP1 一抗 -
描述
兔多克隆抗体to 53BP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human 53BP1 aa 350-450.
Database link: Q12888 -
阳性对照
- IHC-P: Human prostate cancer tissue, mouse renal cancer tissue. WB: HeLa and mouse TCMK1 cell nuclear extract. IP: HEK293T whole cell lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36823于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (4) |
1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
|
IHC-P | (1) |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
IP |
Use at an assay dependent concentration.
|
说明 |
---|
WB
1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa). |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
靶标
-
功能
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
疾病相关
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
序列相似性
Contains 2 BRCT domains. -
翻译后修饰
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
-
数据库链接
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
- Unigene: 481841 Mouse
-
别名
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
图片
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate cancer tissue, labeling CTCF with ab36823 at a 1/5000 dilution. Detection: DAB.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling CTCF with ab36823 at a 1/1000 dilution. Detection: DAB.
-
All lanes : Anti-53BP1 antibody (ab36823) at 0.04 µg/ml
Lane 1 : HeLa cell nuclear extract at 50 µg
Lane 2 : HeLa cell nuclear extract at 5 µg
Lane 3 : TCMK1 mouse cell nuclear extract at 15 µg
Predicted band size: 214 kDa
Exposure time: 30 secondsDetection: Chemiluminescence. -
53BP1 was immunoprecipitated from 1 mg HEK293T whole cell lysate with ab36823 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab243868 at a 1/1000 dilution.
Lane 1: ab36823 IP in HEK293T whole cell lysate.
Lane 2: Contol IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (134)
ab36823 被引用在 134 文献中.
- Magistrati E et al. Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1. EMBO Rep 23:e54160 (2022). PubMed: 34957672
- Feng S et al. RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity. Nat Commun 13:957 (2022). PubMed: 35177609
- Pelicci S et al. Novel Tools to Measure Single Molecules Colocalization in Fluorescence Nanoscopy by Image Cross Correlation Spectroscopy. Nanomaterials (Basel) 12:N/A (2022). PubMed: 35215014
- Whisenant D et al. Transient expression of an adenine base editor corrects the Hutchinson-Gilford progeria syndrome mutation and improves the skin phenotype in mice. Nat Commun 13:3068 (2022). PubMed: 35654881
- Feu S et al. RAD51 is a druggable target that sustains replication fork progression upon DNA replication stress. PLoS One 17:e0266645 (2022). PubMed: 35969531