Anti-5-methylcytosine (5-mC)抗体[33D3] (ab10805)
- 数据表
- 具体的参考文献 (32)
- 实验方案
概述
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产品名称Anti-5-methylcytosine (5-mC)抗体[33D3]
参阅全部 5-methylcytosine (5-mC) 一抗 -
描述小鼠单克隆抗体[33D3] to 5-methylcytosine (5-mC)
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特异性Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine.
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经测试应用适用于: IHC-P, MeDIP, ChIP, IP, Southern Blot, Dot blot, Flow Cyt, IHC-Frmore details
不适用于: ICC -
免疫原
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC).
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常规说明
Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: 99.99% PBS -
Concentration information loading... -
纯度Protein A purified
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Primary antibody说明Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
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克隆单克隆
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克隆编号33D3
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骨髓瘤Sp2/0-Ag14
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同种型IgG1
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轻链类型kappa
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研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab10805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
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| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| MeDIP | Use at an assay dependent concentration. PubMed: 17845077Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. | |
| ChIP | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| Southern Blot | 1/200. | |
| Dot blot | Use at an assay dependent concentration. PubMed: 24386123 | |
| Flow Cyt | Use at an assay dependent concentration. Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). |
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| IHC-Fr | Use at an assay dependent concentration. |
靶标
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形式The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application.
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别名
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
see all
Anti-5-methylcytosine (5-mC) antibody [33D3] 图像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) [33D3] antibody (ab10805)](http://www.abcam.cn/ps/products/10/ab10805/Images/ab10805_review_13478.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) [33D3] antibody (ab10805)This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldigab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.
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![ChIP - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)](http://www.abcam.cn/ps/products/10/ab10805/Images/ab10805-265452-ab10805-chip.jpg)
ChIP - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)This image is courtesy of an anonymous Abreview.Native ChIP analysis using unpurified ab10805 binding 5-methylcytosine (5-mC) in nuclear cell lysate derived from mouse embryonic stem cells. Samples were incubated with primary antibody (2µg/µg chromatin) for 16 hours at 4°C in a Glycerol IP buffer. Protein binding was detected using real-time PCR.
Positive control: Magoh2 in HMT KO cells.
Negative Control: Magoh2 in WT cells.
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![Dot Blot - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)](http://www.abcam.cn/ps/products/10/ab10805/Images/ab10805-261699-10805-DB.JPG)
Dot Blot - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)Image from Nettersheim D et al., PLoS One. 2013;8(12):e82881. Fig S1.; doi: 10.1371/journal.pone.0082881Dot blot carried out using ab10805 (left blot), showing specificity to 5-methylcytosine. Indicated amounts (20–5 ng) of oligonucleotides containing either 5mC (50%) or 5hmC (20 %) were spotted on positive charged nylon membrane and detected either with 5mC (ab10805) or 5hmC antibody.
实验方案
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)参考文献
This product has been referenced in:
- Pereira HS et al. Rye (Secale cereale) supernumerary (B) chromosomes associated with heat tolerance during early stages of male sporogenesis. Ann Bot 119:325-337 (2017). Read more (PubMed: 27818381) »
- Cao Z et al. TSA and BIX-01294 Induced Normal DNA and Histone Methylation and Increased Protein Expression in Porcine Somatic Cell Nuclear Transfer Embryos. PLoS One 12:e0169092 (2017). IHC (PFA fixed) ; Pig . Read more (PubMed: 28114389) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"