Application
Flow Cytometry
Fixation
ethanol 70%
Permeabilization
Yes - tween 5%
Sample
Human Cell (sperm)
Specification
sperm
Gating Strategy
10000
Preparation
Cell harvesting/tissue preparation method: percolgradients
Sample buffer: PBS
Sample buffer: PBS
Other product details
Incubation time
30 minute(s) · Temperature: 27°C · Diluent: PBS_T
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300
Additional data
Additional Notes
(1)Barzideh, J., Scott, R. J. and Aitken, R. J. (2012), Analysis of the global methylation status of human spermatozoa and its association with the tendency of these cells to enter apoptosis. Andrologia. doi: 10.1111/and.12033.
DOI: 10.1111/and.12033
PubMed ID
23121197: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Jaleh Barzideh
Verified customer
提交于 Dec 23 2013