概述

  • 产品名称Anti-5 Lipoxygenase抗体
    参阅全部 5 Lipoxygenase 一抗
  • 描述
    兔多克隆抗体to 5 Lipoxygenase
  • 特异性ab59341 detects endogenous levels of total 5 lipoxygenase protein.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized non phosphopeptide derived from human 5 lipoxygenase around the phosphorylation site of serine 271 (QLSPLE).

  • 阳性对照
    • Human skeletal muscle tissue (IHC) HuvEc cytoplasmic lysate (WB)
  • 常规说明Store at -20° for 1 year

性能

应用

Our Abpromise guarantee covers the use of ab59341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 78 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
  • 通路Lipid metabolism; leukotriene A4 biosynthesis.
  • 序列相似性Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • 翻译后修饰Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
  • 细胞定位Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 LO antibody
    • 5 LOX antibody
    • 5-lipoxygenase antibody
    • 5-LO antibody
    • 5-LOX antibody
    • 5LOX antibody
    • 5LPG antibody
    • ALOX 5 antibody
    • Alox5 antibody
    • Arachidonate 5 lipoxygenase antibody
    • Arachidonate 5-lipoxygenase antibody
    • arachidonic 5-lipoxygenase alpha-10 isoform antibody
    • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-p10 isoform antibody
    • Arachidonic acid 5 lipoxygenase antibody
    • Leukotriene A4 synthase antibody
    • LOG 5 antibody
    • LOG5 antibody
    • LOX5_HUMAN antibody
    • MGC163204 antibody
    see all

Anti-5 Lipoxygenase antibody 图像

  • All lanes : Anti-5 Lipoxygenase antibody (ab59341) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells without immunizing peptide
    Lane 2 : Extracts from HuvEc cells containing immunizing peptide


    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
  • ab59341, at a 1/50 dilution, staining human 5 Lipoxygenase in skeletal muscle in the presence and absence of the immunizing peptide, using Immunohistochemistry, Paraffin embedded tissue.

Anti-5 Lipoxygenase antibody (ab59341)参考文献

This product has been referenced in:
  • Molina-Berríos A  et al. Protective role of acetylsalicylic acid in experimental Trypanosoma cruzi infection: evidence of a 15-epi-lipoxin A4-mediated effect. PLoS Negl Trop Dis 7:e2173 (2013). WB ; Mouse . Read more (PubMed: 23638194) »
  • Jun JI & Lau LF The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing. Nat Cell Biol 12:676-85 (2010). WB ; Human . Read more (PubMed: 20526329) »

See all 2 Publications for this product

Product Wall

Te pido disculpas por la espera.

He confirmado con mis compañeros del laboratorio que la proteína ab114310 puede también utilizarse como control positivo para el anticuerpo ab59341.

Si tuvieras cualquier otra consulta no dudes en co...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"