WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
通路Lipid metabolism; leukotriene A4 biosynthesis.
序列相似性Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
翻译后修饰Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
细胞定位Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.