Anti-5 Lipoxygenase抗体(ab103765)


  • 产品名称Anti-5 Lipoxygenase抗体
    参阅全部 5 Lipoxygenase 一抗
  • 描述
    兔多克隆抗体to 5 Lipoxygenase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human 5 Lipoxygenase aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P09917

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung. This antibody also gave a positive signal within WB in Human Lung and Spleen tissue lysates.



Our Abpromise guarantee covers the use of ab103765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
IHC-P Use a concentration of 1 µg/ml.


  • 功能Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
  • 通路Lipid metabolism; leukotriene A4 biosynthesis.
  • 序列相似性Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • 翻译后修饰Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
  • 细胞定位Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 LO antibody
    • 5 LOX antibody
    • 5-lipoxygenase antibody
    • 5-LO antibody
    • 5-LOX antibody
    • 5LOX antibody
    • 5LPG antibody
    • ALOX 5 antibody
    • Alox5 antibody
    • Arachidonate 5 lipoxygenase antibody
    • Arachidonate 5-lipoxygenase antibody
    • arachidonic 5-lipoxygenase alpha-10 isoform antibody
    • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-p10 isoform antibody
    • Arachidonic acid 5 lipoxygenase antibody
    • Leukotriene A4 synthase antibody
    • LOG 5 antibody
    • LOG5 antibody
    • LOX5_HUMAN antibody
    • MGC163204 antibody
    see all

Anti-5 Lipoxygenase antibody 图像

  • All lanes : Anti-5 Lipoxygenase antibody (ab103765) at 1 µg/ml (Milk blocking 1%)

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa

    Exposure time : 20 minutes

    The expression profile observed is consistent with what has been described in the literature (PMID:20978234).

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab103765 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of 5 Lipoxygenase staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103765, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-5 Lipoxygenase antibody (ab103765)参考文献

ab103765 has not yet been referenced specifically in any publications.

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