46C (小鼠neural progenitor, selected for Sox1 expression cell line)全细胞裂解液(ab27205)


  • 产品名称46C (小鼠neural progenitor, selected for Sox1 expression cell line)全细胞裂解液
  • 常规说明Aliquots are at a concentration of 1-2 mg/ml and contain between 100ug and 200ug of lysate, with a minimum of 100ug. To run on SDS-PAGE under reducing conditions it is necessary for the end user to add reducing agent. Addition of 5% beta-mercaptoethanol is recommended (5ul beta-mercaptoethanol per 100ul lysate).
  • 经测试应用适用于: WBmore details


  • 形式Liquid
  • 存放说明Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液RIPA buffer (Sigma) with added protease inhibitors (Sigma). Nupage 4X LDS sample buffer (Invitrogen) added. The lysates have been boiled. Buffer does not contain a reducing agent.
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 纯化说明Cell lysate from feeder free mouse embryonic stem cells selected for Sox1 expression.
  • 研究领域
  • 背景46C is a cell line, constructed in the laboratory of Austin Smith, in which a drug resistance gene is placed under the control of a Sox1 promoter. The cells used in this lysate were Sox1-positive neural progenitor cells selected using puromycin following growth of Mouse Embryonic Stem Cells in N2B27 neural differentiation media. The Sox1 selection technology is patented.



Our Abpromise guarantee covers the use of ab27205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate 图像

  • A band corresponding to Sox2 was detectable in 46C Sox1-expressing Mouse Neural Progenitor Cell Lysate (ab27205), IOUD2 Oct4-expressing Mouse Embryonic Stem Cell Lysate (ab272020) and F9 Mouse Embryonic Carcinoma Cell Lysate (ab27193). This band was detected in the lysate by Western Blotting using anti-Sox2 antibody ab15830. The Sox2 bands were specifically blocked using the immunising peptide of ab15830. An Oct4 band was also detectable in the 47C lysate by Western Blotting (data not shown). It has not been possible to find a suitable Sox1-specific antibody able to detect Sox1 in 46C by Western Blotting. However, we are confident that 100% of the cells (excluding feeders) that contributed to the lysate were actively expressing Sox1.

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)参考文献

ab27205 has not yet been referenced specifically in any publications.

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We do not sell cell lines, only lysates. Unfortunately I do not know where you can source 46C cell lines. You may try searching online for stem cell providers or consult the literature for which labs have access to...

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