Anti-4 Hydroxynonenal抗体(ab46545)

概述

  • 产品名称
    Anti-4 Hydroxynonenal抗体
    参阅全部 4 Hydroxynonenal 一抗
  • 描述
    兔多克隆抗体to 4 Hydroxynonenal
  • 特异性
    Specifically binds to HNE modified proteins.
  • 经测试应用
    适用于: IHC-Fr, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, a wide range of other species
  • 免疫原

    HNE conjugated to BC.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.0146% EDTA, 0.44% Sodium chloride, 50% Glycerol, 1.23% Sodium phosphate
  • Concentration information loading...
  • 纯化说明
    This antibody was purified by an HNE modified Protein-Sepharose affinity column.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab46545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 24140865
WB Use at an assay dependent concentration. Can be blocked with Hydroxynonenal modified Bovine Serum Albumin (ab194193).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000 - 1/20000.

靶标

  • 相关性
    Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
  • 细胞定位
    Cytoplasmic
  • 别名
    • 4 HNE antibody
    • 4-Hydroxy-2-Nonenal antibody

Anti-4 Hydroxynonenal antibody 图像

  • Image from Wang J et al., PLoS One. 2013;8(1):e53951. Fig 7(A).; doi: 10.1371/journal.pone.0053951.

    Frozen mouse cardiac tissue was homogenized with lysis buffer containing 50 mmol/L Tris-HCl (pH7.5), 5 mmol/L EDTA, 10 mmol/L EGTA, 1X cock tail protease inhibitor, 1X alkaline phosphatase inhibitor and 1X acid phophatase inhibitor, 50 ug/ml phenylmenthysulfonyl fluoride and 1.23 mg/ml Chaps. Extracts were centrifuged at 12000 rpm at 4°C for 15 minutes. 10 ug of the sample proteins was mixed with loading buffer (40 mmol/L Tris-HCl, pH 6.8, 1% SDS, 50 mmol/L DTT, 7.5% glycerol and 0.003% bromophenol blue and heated at 95°C for 5 minutes, and subjected to electrophoresis on a gradient gel (4% to 12%) at 120V. After electrophoresis, the protein was transferred to a PVDF membrane in a transfer buffer. The PVDF membrane was rinsed briefly in TBS buffer containing 50 mM Tris, 137 mM NaCl, pH 7.5 and blocked in buffer (5% milk with 0.5% BSA in TBST buffer (TBS buffer containing 0.1% tween 20) at room temperature for 1 hour. The membrane was then incubated with rabbit anti 4-hydroxy-2-noneal (4HNE) antibody at 1/3000 dilution at 4°C over night, followed by washing three times. The secondary antibody was incubated with the membrane for another one hour at room temperature. Finally the antigen-antibody complexes were visualized with use of an enhanced chemiluminescence kit. Anti-GAPDH (Abcam) was used for normalizing.

     

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling 4-Hydroxynonenal with ab46545 at a dilution of 1/50 incubated for 13 hours at 4°C. Heat mediated antigen retrival was performed with Tris-EDTA at pH 9.0. Permeabilzation was via 0.025% Triton X-100. Blocking was with ab93695 ABC kit at 1% for 20 minutes at room temperature. ab93695 detection kit was used for signal amplification.

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  • Immunohistochemical analysis of Human uterine tissue with intrauterine growth restriction, staining 4 Hydroxynonenal with ab46545 at 1/200 dilution. Staining was detected using AEC.

Anti-4 Hydroxynonenal antibody (ab46545)参考文献

This product has been referenced in:
  • Liu B  et al. Inhibition of ALDH2 by O-GlcNAcylation contributes to the hyperglycemic exacerbation of myocardial ischemia/reperfusion injury. Oncotarget 8:19413-19426 (2017). WB ; Rat . Read more (PubMed: 28038474) »
  • Yang Y  et al. Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats. Mol Med Rep 16:491-498 (2017). Read more (PubMed: 28560422) »

See all 72 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Sol. High pH
Permeabilization
No
Specification
Pancreas
Fixative
Formaldehyde
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提交于 Aug 08 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
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提交于 May 08 2017

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Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
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提交于 Apr 17 2017

Application
IHC - Wholemount
Sample
Mouse Tissue (Intestine organoids)
Specification
Intestine organoids
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提交于 Apr 06 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
70 µg
Specification
Bone tissue (Tibia)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
Yes - 0.025% Triton x-100
Specification
Bone tissue (Femur)
Blocking step
Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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提交于 Sep 11 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle tissue)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount
20 µg
Specification
muscle tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: RT°C
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提交于 Jun 19 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse liver, Rat lung)
Gel Running Conditions
Reduced Denaturing (gel 12 %)
Loading amount
50 µg
Specification
Mouse liver, Rat lung
Blocking step
SlimFast as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 18°C
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提交于 May 05 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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提交于 Feb 27 2015

As I am not experienced in 4-HNE research nor accepted method 4-HNE western blot band densitometry, I must defer to the abReviews and publications that external researches have used ab46545 in. I have listed these below:

abReviews:
http://...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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