概述

  • 产品名称3-Nitrotyrosine ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    1 8 6.9%
    批次间
    样品 n Mean SD CV%
    1 3 13%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    8 ng/ml
  • 范围
    8 ng/ml - 1000 ng/ml
  • 实验步骤Multiple steps standard assay
  • 产品概述

    ab116691 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of 3-nitrotyrosine in cell and tissue lysates. The assay employs an antibody specific for 3-nitrotyrosine coated on a 96-well plate. Standards and samples are pipetted into the wells and 3-nitrotyrosine present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-3-nitrotyrosine detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of 3-nitrotyrosine bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.


     


    All species.

  • 说明

    Store all components at 4°C. This kit is stable for 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab116691 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    3-Nitrotyrosine ELISA Kit 图像

    • Example standard curve.

    实验方案

    3-Nitrotyrosine ELISA Kit (ab116691)参考文献

    This product has been referenced in:
    • Walsh LK  et al. Administration of tauroursodeoxycholic acid prevents endothelial dysfunction caused by an oral glucose load. Clin Sci (Lond) 130:1881-8 (2016). Read more (PubMed: 27503949) »
    • Jang JE  et al. Nitric Oxide Produced by Macrophages Inhibits Adipocyte Differentiation and Promotes Profibrogenic Responses in Preadipocytes to Induce Adipose Tissue Fibrosis. Diabetes 65:2516-28 (2016). Sandwich ELISA . Read more (PubMed: 27246913) »

    See all 7 Publications for this product

    Product Wall



    3-nitrotyrosine is a chemical formed in vivo by the reaction of tyrosine with nitrating oxidants, superoxide and nitric acid (http://www.ncbi.nlm.nih.gov/pubmed/1416975) with the chemical formula C9H10N2O5. It's structure does not change betw...

    Read More

    Thank you for your enquiry.



    Lung is a great sample to run with this assay!


    My general recommendation is to homogenize and then sonicate the tissue. Sonicate until the sample is not sticky - DNA released from ruptured...

    Read More

    Thank you for contacting us.

    The competitive ELISA is considered to be more sensitive than the sandwich ELISA. Both are compatible with your samples

    Please do not hesitate to contact us if you need any more advice or information. ...

    Read More

    Thank you for your reply.


    Our recommendation would be to collect samples using the extraction buffer provided with the kit. Without knowing the recipe of the buffer it is impossible to say whether or not it is compatible. Please let me kn...

    Read More

    Thank you for your reply.

    Regarding your results - can you provide the formulation of the lysis buffer you are using? As the controls and standards are working, I expect the problem is with the sample preparation. Can you also provide your dat...

    Read More

    Thank you for contacting Abcam regarding ab116691.

    Unfortunately we do not sell these components separately. The kits are designed to provide sufficient amounts of reagents for 1 96 well plate, so in order to acquire additional strips, you wou...

    Read More

    Thank you for your enquiry regarding ab116691.

    3-nitrotyrosine BSA standard is from purified BSA.

    I hope this information helps you, and please do not hesitate to contact us if you have further questions.

    Thank you for contacting us.

    Here is the response from the lab:

    As far as we know we haven’t tested this kiton plasma samples. There is no reason that it is not possible, but the question is primarily one of sensitivity (are ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"