3-Nitrotyrosine ELISA试剂盒(ab116691)
Key features and details
- Sensitivity: 8 ng/ml
- Range: 8 ng/ml - 1000 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
概述
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产品名称
3-Nitrotyrosine ELISA试剂盒
参阅全部 Nitrotyrosine 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 8 6.9% 批次间 样品 n Mean SD CV% 1 3 13% -
样品类型
Cell culture extracts, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
8 ng/ml -
范围
8 ng/ml - 1000 ng/ml -
实验步骤
Multiple steps standard assay -
产品概述
ab116691 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of 3-nitrotyrosine in cell and tissue lysates. The assay employs an antibody specific for 3-nitrotyrosine coated on a 96-well plate. Standards and samples are pipetted into the wells and 3-nitrotyrosine present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-3-nitrotyrosine detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of 3-nitrotyrosine bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
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说明
Store all components at 4°C. This kit is stable for 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X 3-nitrotyrosine Detector Antibody 1 x 1ml 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 20X Buffer 1 x 20ml 3-nitrotyrosine BSA standard (Lyophilized) 1 vial Extraction Buffer (ab260490) 1 x 15ml Microplate 96 antibody coated wells in 12 strips 1 unit HRP Development Solution 1 x 12ml -
研究领域
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相关性
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
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数据表及文件
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SDS download
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Datasheet download
文献 (62)
ab116691 被引用在 62 文献中.
- Pérez-Castro CC et al. Hyperbaric oxygenation applied before or after mild or hard stress: effects on the redox state in the muscle tissue. Korean J Physiol Pharmacol 27:9-20 (2023). PubMed: 36575929
- Guo C et al. MicroRNA-214-5p aggravates sepsis-related acute kidney injury in mice. Drug Dev Res 83:339-350 (2022). PubMed: 34370322
- Forsse JS et al. The Influence of an Acute Bout of Aerobic Exercise on Vascular Endothelial Function in Moderate Stages of Chronic Kidney Disease. Life (Basel) 12:N/A (2022). PubMed: 35054484
- Xie W et al. Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance. Burns Trauma 10:tkac007 (2022). PubMed: 35415192
- Zhang ZZ et al. MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway. Oxid Med Cell Longev 2022:8659587 (2022). PubMed: 35602094