Anti-3-Nitrotyrosine抗体[39B6] (ab61392)

概述

  • 产品名称
    Anti-3-Nitrotyrosine抗体[39B6]
    参阅全部 3-Nitrotyrosine 一抗
  • 描述
    小鼠单克隆抗体[39B6] to 3-Nitrotyrosine
  • 宿主
    Mouse
  • 特异性
    No detectable crossreactivity with non-nitrated tyrosine. Not species specific.
  • 经测试应用
    适用于: IHC-P, IHC-Fr, IHC-FoFr, WB, ELISA, IPmore details
  • 免疫原

    3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.

  • 阳性对照
    • SIN-1 (3-morpholinosydnonimine) treated BSA. Use between 1-2.5mM SIN-1 for 1-24 hours, grow cells under normal growth conditions. Please see Yasuda S, Idell S, Liu MC. Biochem J. 2007 Jan 15;401(2):497-503. PMID: 17002600.

性能

应用

Our Abpromise guarantee covers the use of ab61392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20708681
WB 1/1400.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 相关性
    Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
  • 别名
    • Nitrotyrosine antibody

图片

  • All lanes : Anti-3-Nitrotyrosine antibody [39B6] (ab61392) at 1/3000 dilution

    All lanes : Mouse skeletal muscle

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse HRP conjugated at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 4 seconds


    Blocked with 5% milk for 2 hours.

    See Abreview

  • ab61392 staining 3-nitrotyrosine in Bouin's fixed paraffin-embedded backskin sections of transgenic mice.

    Blocker: 5% goat serum in buffer. Buffer: 99% washing buffer (7g BSA and 222ul gelatin topped up to 1L dH2O), 1% heat inactivated goat serum. Primary Dilution: 1/100 for 1 hour at room temperature. Secondary: FITC-conjugated goat anti-mouse IgG, 1/50 for 1 hour at room temperature.

  • ab61392 staining of Nitrotyrosine residues at 1/100 in rat liver formaldehyde fixed sections three days after zymosan treatment. Staining was detected with a fluorescein secondary antibody and counterstained with DAPI.

文献

This product has been referenced in:
  • Hao L  et al. Mitochondria-targeted ubiquinone (MitoQ) enhances acetaldehyde clearance by reversing alcohol-induced posttranslational modification of aldehyde dehydrogenase 2: A molecular mechanism of protection against alcoholic liver disease. Redox Biol 14:626-636 (2018). WB, IHC-P ; Mouse . Read more (PubMed: 29156373) »
  • García-Ruiz I  et al. Omentectomy Prevents Metabolic Syndrome By Reducing Appetite and Body Weight In A Diet-Induced Obesity Rat Model. Sci Rep 8:1540 (2018). WB . Read more (PubMed: 29367725) »

See all 44 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate buffer (pH 6.0)
Permeabilization
Yes - 0.4% Triton X-100
Specification
liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 18 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Iliac vessel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Iliac vessel
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 27 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skin)
Gel Running Conditions
Reduced Denaturing (10% Gel)
Loading amount
50 µg
Treatment
Cinnamaldehyde
Specification
Skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 10 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle (GTN))
Specification
Skeletal muscle (GTN)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 09 2014

The protocol you provided is for the IHC on rat brain, which you say showed some background staining but nothing that appeared specific. Did you have a treated/diseased rat brain for comparison? Did you include a negative control, using no primary anti...

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Thank you for contacting us.

I am not aware of a reference using this antibody on human skin. On the datasheet you have an image of frozen section mouse skin. I am sorry that I can not help you more on this occasion.

Please be also...

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Thanks for your email.
I believe this replacement order has been shipped and delivered on Tuesday July 18. It was on Abcam order reference number 1128916 which contained a vial of ab61392.
I hope this is helpful. Please let me know if you hav...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab61392 and one vial of ab52818.

To check the status of th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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