Anti-3-Nitrotyrosine抗体[11C2] (ab53232)

概述

  • 产品名称Anti-3-Nitrotyrosine抗体[11C2]
    参阅全部 3-Nitrotyrosine 一抗
  • 描述
    小鼠单克隆抗体[11C2] to 3-Nitrotyrosine
  • 特异性This antibody reacts with free and bound Nitrotyrosine.
  • 经测试应用适用于: WB, ELISA, ICC, IHC-Pmore details
  • 免疫原

    Chemical/ Small Molecule: Nitrotyrosine conjugated to KLH.

性能

应用

Our Abpromise guarantee covers the use of ab53232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500.
ELISA 1/100 - 1/1000.
ICC 1/100 - 1/500.
IHC-P 1/250. PubMed: 19541933

靶标

  • 相关性Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
  • 别名
    • Nitrotyrosine antibody

Anti-3-Nitrotyrosine antibody [11C2] (ab53232)参考文献

This product has been referenced in:
  • Guix FX  et al. Modification of ?-secretase by nitrosative stress links neuronal ageing to sporadic Alzheimer's disease. EMBO Mol Med : (2012). WB . Read more (PubMed: 22488900) »
  • McGrath-Morrow S  et al. Nrf2 increases survival and attenuates alveolar growth inhibition in neonatal mice exposed to hyperoxia. Am J Physiol Lung Cell Mol Physiol 296:L565-73 (2009). IHC-P . Read more (PubMed: 19151108) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us. The note on the nitrotyrosine antibody datasheet stating a predicted molecular weight of 1 kDa is incorrect with regards to what one can expect to see in a western blot. Isolated nitrotyrosine may itself be about 1 kDa but ...

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Thank you for your enquiry. I am sorry to confirm that non of our Oxoguanine 8, Nitrotyrosine, and Malondialdehyde antibodies have been tested with half Karnovsky's fixative. Regrettably, this means we therefore cannot confirm and guarantee how well...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"