17 OH Progesterone ELISA试剂盒(ab108668)

概述

• 产品名称
  17 OH Progesterone ELISA试剂盒
• 检测方法
  Colorimetric
• 精确度

  批次内

  样品	n	Mean	SD	CV%
  Control Sera	3			<=8.2%

  批次间

  样品	n	Mean	SD	CV%
  Control Sera	3			<=13.8%

• 样品类型
  Serum, Plasma
• 检测类型
  Competitive
• 灵敏度
  = 0.05 ng/ml
• 复检

  98.66 %
  

• 实验步骤
  Multiple steps standard assay
• 产品概述

  Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.

   

  A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.

• 经测试应用
  适用于: Competitive ELISAmore details
• 平台
  Microplate

性能

• 存放说明
  Store at +4°C. Please refer to protocols.

• 组件	1 x 96 tests
  10X Wash Solution	1 x 50ml
  17 OH Progesterone Control	1 x 1ml
  17 OH Progesterone Standard 0 – 0 ng/mL	1 x 1ml
  17 OH Progesterone Standard 1 – 0.2 ng/mL	1 x 1ml
  17 OH Progesterone Standard 2 – 0.6 ng/mL	1 x 1ml
  17 OH Progesterone Standard 3 – 2.0 ng/mL	1 x 1ml
  17 OH Progesterone Standard 4 – 6.0 ng/mL	1 x 1ml
  17 OH Progesterone Standard 5 – 20.0 ng/mL	1 x 1ml
  17 OH Progesterone-HRP Conjugate	1 x 22ml
  Anti-17 OH Progesterone IgG Coated Microplate (12 x 8 wells)	1 unit
  Cover foils	1 unit
  Stop Solution	1 x 15ml
  Strip holder	1 unit
  TMB Substrate Solution	1 x 15ml

• 研究领域
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Progesterone

  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Progesterone

  • Developmental Biology
  • Reproduction
  • Hormones

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Growth factors and hormones ELISA kits

17 OH Progesterone ELISA Kit 图像

• 

  Typical Standard Curve

  Representative Standard Curve using ab108668