17 OH Progesterone ELISA试剂盒(ab108668)

概述

  • 产品名称17 OH Progesterone ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Control Sera 3 <=8.2%
    批次间
    样品 n Mean SD CV%
    Control Sera 3 <=13.8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.05 ng/ml
  • 复检

    98.66 %

  • 实验步骤Multiple steps standard assay
  • 产品概述

    Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.

     

    A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab108668 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Competitive ELISA Use at an assay dependent dilution.

    17 OH Progesterone ELISA Kit 图像

    • Representative Standard Curve using ab108668

    实验方案

    17 OH Progesterone ELISA Kit (ab108668)参考文献

    ab108668 has not yet been referenced specifically in any publications.

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