17 OH Progesterone ELISA试剂盒(ab108668)
Key features and details
- Sensitivity: 0.05 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
概述
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产品名称
17 OH Progesterone ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Control Sera 3 <=8.2% 批次间 样品 n Mean SD CV% Control Sera 3 <=13.8% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
= 0.05 ng/ml -
回收率
98.66 %
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实验步骤
Multiple steps standard assay -
产品概述
Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Solution 1 x 50ml 17 OH Progesterone Control 1 x 1ml 17 OH Progesterone Standard 0 – 0 ng/mL 1 x 1ml 17 OH Progesterone Standard 1 – 0.2 ng/mL 1 x 1ml 17 OH Progesterone Standard 2 – 0.6 ng/mL 1 x 1ml 17 OH Progesterone Standard 3 – 2.0 ng/mL 1 x 1ml 17 OH Progesterone Standard 4 – 6.0 ng/mL 1 x 1ml 17 OH Progesterone Standard 5 – 20.0 ng/mL 1 x 1ml 17 OH Progesterone-HRP Conjugate 1 x 22ml Anti-17 OH Progesterone IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108668 被引用在 1 文献中.
- Fu Q et al. Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development. Int J Mol Sci 17:N/A (2016). PubMed: 27136540