RabMAb

Anti-160 kD Neurofilament Medium (phospho S614 + S619)抗体[EPR580(2)Y] (ab68142)

概述

  • 产品名称Anti-160 kD Neurofilament Medium (phospho S614 + S619)抗体[EPR580(2)Y]
    参阅全部 160 kD Neurofilament Medium 一抗
  • 描述
    兔单克隆抗体[EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human 160 kD Neurofilament Medium

  • 阳性对照
    • SH-SY5Y cell lysates; human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab68142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: 1/100 - 1/250.
    WB: 1/500. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa).

    Is unsuitable for Flow Cyt, ICC or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
    • 细胞定位Cytoplasm
    • 数据库链接
    • 别名
      • 150kDa medium antibody
      • NEF3 antibody
      • NEFM antibody
      • Neurofilament 3 antibody
      • NF160 antibody
      • NFM antibody
      see all

    Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] 图像

    • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68142 at a dilution of 1/100 - 1/250
    • All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142) at 1/500 dilution

      Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase
      Lane 2 : Untreated SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)参考文献

    ab68142 has not yet been referenced specifically in any publications.

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